Fig. 4
From: USP27-mediated Cyclin E stabilization drives cell cycle progression and hepatocellular tumorigenesis
USP27 regulates cell cycle progression and cell proliferation. a Hep3B cells stably expressing control or USP27 knockdown plasmids were synchronized at G2/M stage by treatment of nocodazole (50 ng/ml) twice for 20 h separately and then released into fresh medium and analyzed by western blot. b, c The effects of USP27 knockdown, USP27 knockdown plus Cyclin E overexpression b or single overexpression of USP27 c together with their respective controls on Hep3B cell cycle progression were measured by flow cytometry analysis. d–f The growth of Hep3B d, f or MHCC97H e cells stably expressing shUSP27, shUSP27 + Cyclin E, USP27, or their corresponding control vector was examined
