Fig. 5
From: USP27-mediated Cyclin E stabilization drives cell cycle progression and hepatocellular tumorigenesis
Suppression of USP27 expression delayed tumor growth. a Hep3B cells stably expressing control shRNA, shRNA against USP27 (shUSP27), Cyclin E overexpression plasmids plus shUSP27 (shUSP27 + Cyclin E), or double knockdown plasmids of Cyclin E and USP27 (shUSP27 + shCyclin E) were seeded in soft agar and cultured for 3–4 weeks. Colonies were visualized by light microscopy and quantified. b MHCC97H cells stably expressing indicated plasmids were seeded in soft agar and the numbers of colonies were quantified as in a. c Hep3B cells stably expressing indicated plasmids were seeded in soft agar and the numbers of colonies were quantified as in a. d–f Hep3B cells stably expressing control, single knockdown of USP27 or double knockdown of USP27 and Cyclin E plasmids were inoculated subcutaneously into nude mice. Six weeks after injection, mice were killed, photographed, and weighed. Tumor size was measured every week
