Fig. 5
From: A modified gene trap approach for improved high-throughput cancer drug discovery
Characterization of vemurafenib resistant SABRE line. a Comparison of the vemurafenib resistant SB01-VR cells (red line) to the SB01 parental cells (black line). Viability curves show a 60-fold increase in IC50 value in the SB01-VR cells compared to the SB01 cells after 72 h vemurafenib treatment. b Following 2 h incubation with the indicated concentrations of vemurafenib, significant levels of ERK1/2 phosphorylation can still be detected by western blot analysis in the SB01-VR line, as compared to the parental vemurafenib sensitive SB01 line (20 μg per lane). c–e 96-well plate assays to assess luciferase activity in the SB01-VR line in response to various drug treatments. NR, no response. Error bars ± SD
