Fig. 2 | Oncogene

Fig. 2

From: C5a induces A549 cell proliferation of non-small cell lung cancer via GDF15 gene activation mediated by GCN5-dependent KLF5 acetylation

Fig. 2

C5aR expression in human NSCLC cell lines as well as cell proliferation and KLF5, GCN5, GDF15 expression upon C5a stimulation. a IB assay showed that human C5aR was highly expressed in A549 and PC9 cell lines. b–d The proliferation of A549 and PC9 cells exposed to C5a (25 ng/ml and 250 ng/ml, especially 25 ng/ml) was notably increased by CCK8 (72 h, b), cell counting (7 days, c), and colony-forming assays (14 days, d). e, f Real-time PCR (e) and IB (f) exhibited that mRNA and protein of KLF5, GCN5, and GDF15 in A549 cells following 25 ng/ml C5a incubation started to increase from 1 h, and peaked at 2 h and 3 h (GCN5 peaked at 6 h). Representative photographs are manifested. Data are expressed as means ± S.E.M. from three independent experiments, *P < 0.05, **P < 0.01 vs. 0 ng/ml C5a

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