Fig. 2
From: Cross-species genomics identifies DLG2 as a tumor suppressor in osteosarcoma
DLG2 restoration hinders cell and tumor growth in in vitro 3D cultures and xenograft experiments. a Representative pictures of colonies in 3D-matrigel. DLG2 re-expression induced a significant reduction in both colony number and size. Quantification of colony area is shown on the right. b Representative soft agar images and their respective quantifications. DLG2 restoration reduces colony forming capacities. c NOD-SCID mice injected with luciferase- expressing SJSA-1 parental (left) and DLG2 (right) cells imaged at 3 weeks post injection (n = 10 per group). Photon influx value was used as a surrogate marker for tumor size. Photon influx of each tumor was quantified and average values are shown on the right. d Tumors taken from NOD-SCID mice 4 weeks after innoculation with OVC-COSA-31 parental (left) and DLG2 (right) canine OS cells (n = 10 per group). Tumor size was calculated and is shown on the right. White bar represents 1 cm. Values shown are the mean ± SD of three separate determinations. *P < 0.05 by two-tailed Student’s t test
