Fig. 2
An overview of the transcriptional landscape of Snail-regulated EMP. a Phase-contrast images of LNCaP–iSnail cells treated with Dox for 5 days to induce EMT and subsequently removed to allow for MErT over 20 days. b Heatmap representation showing the expression of genes altered at any time during the 5 day EMT and 3, 5, and 20 days of MErT (5157 gene identities can be found in the supplementary dataset 1). c Heatmap representation showing the expression of known epithelial and mesenchymal genes in LNCaP–iSnail and LNCaP–iGFP cells treated with Dox for 5 days followed by removal for 3, 5, and 20 days. d qRTPCR validation of CDH1, EpCAM, ZEB1, SNAI1, VIM, and CTGF. Gene expression was normalised to RPL32. Fold change is relative to untreated LNCaP–iSnail cells (NoDox). One-way ANOVA, *p < 0.05. Error bars indicate SEM for biological triplicates. e Six transcriptional clusters were generated through pattern matching of select genes (blue line) and classified by temporal reversion pattern. Clusters I and II show reverting expression by day 3 of MErT (MErT3; M3); Clusters III and IV by day 5 (MErT5; M5); and Clusters V and VI by day 20 (MErT20; M20). Cluster avg is the average transcriptional pattern of the transcript cluster. Heatmap representation showing the gene expression of the genes within each of the three cluster pairs. Heatmap color for all heatmaps refers to normalised z-score
