Fig. 1

Identification of androgen-stabilised proteome in prostate cancer (PrCa). a Scatterplot showing the relationship between protein levels following AR knockdown and AR antagonist treatment of C4-2 cells grown in full media. Red line shows lowess regression, red points show commonly downregulated proteins, light blue points show commonly upregulated proteins (cor = 0.51). PBK is shown in green. b Western blot analysis showing of expression of PBK and AR proteins. C4-2 cells were transiently transfected with small interfering (si)-RNA targeting AR (siAR) or with non-targeting control siRNA (siNT); β-actin is the loading control for AR and the Ponceau for PBK. c Immunohistochemical staining score (H score) of nuclear PBK protein expression in tumours (in triplicates) and untreated benign adjacent epithelia (in duplicates) in patients with (n = 27) or without (n = 20) 7 days’ treatment with the LHRH analogue degarelix, statistical significance calculated by Mann–Whitney test. d Representative IHC images of corresponding tissue samples from degarelix-treated and untreated patients. Scale bars = 50 µm. H score ranged from 0 to 9 and converted it to a 4-point scale as 0 = none, 1–3 = weak; 4–6 = moderate and 7–9 = strong