Fig. 5

TAT-TROY (234–371 aa) protein disrupts the interaction between TROY and RKIP. (a) Direct interaction of RKIP with TROY by GST pull-down assay. (b) Schematic representation of truncation mutants of HA-TROY and their interaction with GFP-RKIP in co-transfected HEK293T cells. (c) The direct interaction between GST-TROY and His-RKIP were inhibited after incubation with TAT-TROY (234–371 aa). (d) The band intensities of c are quantified. The quantification of the precipitates are normalized to those of lysate. The treated group are normalized to the control. (e) TAT-mediated internalization. U87 cells were incubated with 0.2 μM of TAT-TROY (234–371 aa) or the PBS (control) for 20 min at 37℃. TAT fusion protein was labelled by anti-TAT antibody (green). (f) The interaction of RKIP/TROY in U87 cells was significantly inhibited after TAT-TROY (234–371 aa) protein treatment. (g) The band intensities of f are quantified. The quantification of the precipitates are normalized to those of lysates. The treated group are normalized to the control. **P < 0.01