Fig. 4 | Oncogene

Fig. 4

From: CBAP modulates Akt-dependent TSC2 phosphorylation to promote Rheb-mTORC1 signaling and growth of T-cell acute lymphoblastic leukemia

Fig. 4

CBAP regulates upstream of Ras/Rac1 activation and TSC2-mTORC1 signaling cascades in Jurkat cells. a, b Activated Ras (a) and activated Rac1 (b) were precipitated by performing Ras-RBD and Rac1-PBD pull-down assays, respectively, and were detected by western blot using specific antibodies. Total cell lysate was pre-loaded with GTPγS or GDP as positive and negative controls, respectively, in the assays. c Tyrosine phosphorylation of PI3Kp85. Lysates were immunoprecipitated with anti-phosphotyrosine (pTyr) antibody before being subjected to Western blot using anti-PI3Kp85 antibody. d–f Immunoblotting analysis of signaling molecules in cr-Ctrl and leukemic CBAP-KO Jurkat cells in response to treatment with MK2206 (d), U0126 (e), or co-treatment with MK2206 and U0126 (f). g Schematic representation of the crosstalk between the Raf-MEK-ERK–RSK and Akt-TSC2-p70S6K pathways and the effects of CBAP knockout and inhibitors on the Jurkat cell line

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