Fig. 6

Inhibition of miR-10b and miR-222 induced cell cycle arrest and apoptosis of GBM cells, and BIM was co-targeted by miR-10b and miR-222. a The LN229, U87MG, and U251 cells were transfected with indicated miRNA inhibitors for 72 h, and the promotion of apoptosis was measured by AnnexinV‐FITC/PI double staining. b Luciferase reporter assays in glioma cells after co-transfection of cells with B1, B2, and B3 plasmids and miRNA mimics. The data were represented as mean ± s.d. (n = 3), *p < 0.01 versus vector group for each plasmid. c Western blot and RT-PCR analysis for BIM in LN229, U251, and U87MG. d Cell cycle analysis of miR-Scr and As-miR-10b/222 transfected cells in LN229, and overview of the cell cycle. e Western blot analysis for p21 and p27 in U251 and U87MG after transfection as indicated