Fig. 1

IL-33 and ST2L are overexpressed in human GC tissues and predict poor prognosis. a QRT-PCR analysis of IL-33 mRNA expression in GC and corresponding normal tissues (n = 18). b Histogram displaying the relative mRNA expression of IL-33 in 18 GC tissues. Data are shown as −ΔΔCt and 2^−ΔCt. c IHC staining of α-SMA, IL-33 and ST2L in GC tissues (200×; scale bar = 100 μm). d Histogram displaying the number of α-SMA, IL-33 and ST2L positive cells/field in GC tissues. e Histogram displaying the correlation between IL-33 expression and ST2L expression determined by IHC (r = 0.6503). f, g Survival of patients with low or high levels of IL-33 and ST2L protein expression. h–j Immunofluorescence staining of IL-33, α-SMA, FAP, ST2L and Cytokeratin in GC tissues (200×; scale bar = 400 μm). k–m Histogram displaying the percentage of IL-33⁺/α-SMA⁺, IL-33⁺/FAP⁺ and ST2L⁺/Cytokeratin⁺⁺ cells in GC tissues. Data are represented as the mean ± SD; *P <0.05, **P <0.01, ***P <0.001