Fig. 1: The expression of STAP-1 aberrantly increased in human CML LSC subset.

a, b BM cells were collected from healthy donor (n = 7) and newly diagnosed chronic CML patients (n = 8), and Lin⁻ CD34⁺ CD38⁻ hematopoietic stem cells and Lin⁻ CD34⁺ CD38⁺ hematopoietic progenitor cells were sorted using flow cytometry. a The representative data of flow cytometry are shown. b STAP-1 and STAP-2 mRNA were measured by real-time RT-PCR in the indicated subsets. Bar graph shows expression levels of STAP-1 and STAP-2 mRNAs normalized to data from MNC fraction of healthy donor. c Lin⁻ CD34⁺ BM cells from newly diagnosed chronic CML patients (n = 6) were transduced with control or STAP-1 shRNA lentivirus, cultured in triplicates in methylcellulose medium for 14 days, and colonies were counted. Data are shown as mean ± SD. *p < 0.05 and **p < 0.01 indicate significant difference. MNC mononuclear cells, NS not significant.