Fig. 5: STAP-1 affects JAK/STAT signaling as well as PPAR signaling pathways.

Gene expression profiles of CML LSCs at day 11 after first BMT between WT and STAP-1 KO mice were compared by RNA-seq analysis. a Scatter plot represents the mean of normalized counts. In all, 3792 genes (2536 downregulated genes; 1256 upregulated genes) were differentially expressed (twofold change) in STAP-1 KO LSCs (y-axis) relative to WT LSCs (x-axis). b–d Gene expression data were subjected to bioinformatics analysis by Ingenuity Pathway Analysis. For each signaling pathway, an enrichment p value and a z-score of activation were calculated. Pathways that were predicted to be downregulated or upregulated in STAP-1 KO CML LSCs compared with WT CML LSCs are shown in (b). Pathways involved in inflammatory responses are shown in (c). Upstream regulatory candidates are shown in (d). Candidates of interest are labeled.