Fig. 1: COPZ1 expression is elevated in primary human glioma samples and GBM cell lines.
From: Loss of COPZ1 induces NCOA4 mediated autophagy and ferroptosis in glioblastoma cell lines
a COPZ1 RNA expression (log2) based on the 2016 WHO classification from the TCGA database. b Kaplan–Meier survival analysis of patient overall survival data based on high versus low expression of COPZ1 from the TCGA dataset. c Representative western blot of protein levels of COPZ1 in lysates (20 µg) prepared from human glioma tissues (n = 9) and non-neoplastic brain tissues (n = 3). d Relative quantification of western blots shown in (c). e Representative images of IHC staining for COPZ1 in human glioma and non-neoplastic brain tissue samples (Normal, n = 6; WHO II, n = 18; WHO III, n = 18; WHO IV, n = 18). Scale bar for the upper images, 50 µm and the lower images, 100 µm. f Graphical results showing the IHC scores. g Representative western blot of COPZ1 protein levels in normal human astrocytes (NHA) and human GBM cell lines. h Relative quantification of western blots shown in (g). i Immunofluorescence staining of COPZ1 (green) in U87MG and U251 cells analyzed with fluorescence microscopy. The nuclei were stained with DAPI (blue), while the cytoskeleton was stained with pholloidin (red). Scale bar, 10 µm. One-way ANOVA for multi-group comparisons: *p < 0.05, **p < 0.01, ***p < 0.001; log-rank test: p < 0.05.
