Fig. 4: Intracellular iron increases with loss of COPZ1 and enhances cell death by causing ROS and lipid peroxidation.
From: Loss of COPZ1 induces NCOA4 mediated autophagy and ferroptosis in glioblastoma cell lines
a MDA levels detected in U87MG, U251 and P3#GBM glioma cells transfected with si-COPZ1# for 48 h. b MDA levels detected in si-COPZ1#1 transfected cells pretreated with DFO, Fer-1, FAC, or GSH. c Iron assay to detect ferrous iron levels in the presence of DFO. d LDH release assay of transfected cells treated with DFO, Fer-1, or FAC. e Hydrogen peroxide assay showing accumulation of H2O2 in P3#GBM cells transfected with si-COPZ1#1 and treated with DFO, FAC and Fer-1 relative to controls. f Representative images of dihydroethidium (DHE; red fluorescence) superoxide probe 48 h after transfection of cells with si-COPZ1#1. For each group of 2 cell lines, 3 images from triplicate experiments were counted. Scale bar, 75 μm. g Statistical analysis of the fluorescence intensities of U87MG and the U251 GBM cell lines transfected with si-COPZ1#1 compared to their respective control cells. Student’s t test for two-group comparison: *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA for multi-group comparisons: NS = non-significant, *p < 0.05, **p < 0.01, ***p < 0.001.
