Fig. 7: Down-regulation of COPZ1 reduces in vivo tumor growth.
From: Loss of COPZ1 induces NCOA4 mediated autophagy and ferroptosis in glioblastoma cell lines
a Images of Intracranial tumor growth of luciferase expressing U87MG-sh-COPZ1#1 cells or U87MG-NC cells monitored at days 7, 14, and 21 after implantation using the IVIS-200 imaging system to detect bioluminescence. b Quantification of the bioluminescent signals from the orthotopic tumors in mice implanted with U87MG-sh-COPZ1#1 cells or U87MG-NC cells at days 7, 14, and 21. c Kaplan–Meier analysis of overall survival of tumor bearing animals. A log-rank test was used to assess the statistical significance of the differences. d Representative images of hematoxylin and eosin-stained sections from brains of orthotopic U87MG-sh-COPZ1#1 or U87MG-NC tumor bearing nude mice. Scale bar, 100 µm. e Representative images of immunohistochemical staining for NCOA4 and Ki67 in sections from brains of orthotopic U87MG-sh-COPZ1#1 or U87MG-NC tumor bearing nude mice. Scale bar third column, 100 μm; scale bar fourth column, 25 μm. f Comparison of ferrous iron levels in orthotopic U87MG-sh-COPZ1#1 or U87MG-NC xenograft samples. g Comparison of MDA levels in orthotopic U87MG-sh-COPZ1#1 or U87MG-NC xenograft samples. h Schematic figure of the COPZ1 induction of ferroptosis in GBM. Student’s t test for two-group comparison: *p < 0.05, **p < 0.01, ***p < 0.001; log-rank test: p < 0.05.
