Fig. 7: WFDC21P inhibits cell proliferation and tumorigenesis via PFKP and PKM2.

a WFDC21P cannot influence cell proliferation in PFKP-knockdown cells. WFDC21P was knocked down in control Huh7 cells or Huh7 cells with silenced PFKP. MTT assays (left) and cell counting analysis (right) were carried out. b WFDC21P cannot inhibit glucose uptake and lactate production in PFKP-knockdown cells. Glucose uptake (left) and lactate production (right) were detected in cells descripted in a. c WFDC21P cannot inhibit tumor growth in xenografts derived from cells with PFKP knocked down. The tumor growth curves, tumor weights and Ki-67 expression levels are shown. Scale bars in xenograft images and Ki-67 staining were 1 cm and 100 μm, respectively. d WFDC21P cannot influence cell proliferation in PKM2-knockdown cells. e WFDC21P cannot inhibit glucose uptake and lactate production in PKM2-knockdown cells. f WFDC21P cannot inhibit tumor growth in xenografts derived from cells with PKM2 knocked down. The tumor growth curves, tumor weights and Ki-67 expression levels are shown. Scale bars in xenograft images and Ki-67 staining were 1 cm and 100 μm, respectively. The data are represented as the means ± SEM of at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 and ns no significance.