Fig. 3: B-Myb promotes cell cycle progression in colorectal cancer cells. | Oncogene

Fig. 3: B-Myb promotes cell cycle progression in colorectal cancer cells.

From: B-Myb accelerates colorectal cancer progression through reciprocal feed-forward transactivation of E2F2

Fig. 3

a–d Cell cycle distribution. The stable B-Myb overexpression or knockdown cells were seeded on six-well plates, and 24 h later, cells were collected and subjected to flow cytometer analysis. e, f BrdU labeling. DNA biosynthesis was labeled by BrdU (red), and DAPI was used to stain cell nuclei (blue) in the stable B-Myb overexpression or knockdown cells. BrdU positive cells were counted and subjected to statistical analysis. g The effect of B-Myb overexpression on mitosis. The stable B-Myb overexpression cells were fixed and stained with anti-phospho-histone H3 (pHH3) antibody (red). The pHH3-positive cells were counted and subjected to statistical analysis. Data represent the mean ± SD. All experiments were performed in triplicates. *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page