Fig. 1: EWS cell lines showed a remarkably sensitivity to selective HDAC6 inhibition. | Oncogene

Fig. 1: EWS cell lines showed a remarkably sensitivity to selective HDAC6 inhibition.

From: Selective inhibition of HDAC6 regulates expression of the oncogenic driver EWSR1-FLI1 through the EWSR1 promoter in Ewing sarcoma

Fig. 1

A Cell viability assessment by 50% inhibitory concentration (IC50) of 13 EWS cell lines, one non-tumor cell line, and 5 non-EWS cancer cell lines, after 72 h exposure to BML-281. B Statistical comparison of the average sensitivity to IC50 HDAC6 inhibition between the two cell lines groups. C A schematic representation of the performed protocol for EWSR1-FLI1 induction (upper left panel), and confirmation of the EWSR1-FLI1 ectopic expression protein by western blotting using HeLa cells treated with doxycycline (lower left panel). Numbers below blots represent densitometric quantification of bands, normalized to endogenous bands. Statistical comparison of sensitivity to HDAC6 inhibition with ectopic EWSR1-FLI1 protein expression and no expression in two clones with inducible chimeric protein-expression system in HeLa cells (right panel). D Cleaved-caspase 3 level assessment in SKNMC and WE68 cells treated with BML-281 in dose-dependent manner for 48 h and analyzed by flow cytometry. E Effects of HDAC6 inhibition on the anchorage-independent growth capacity of the EWS cell lines (SKNMC and WE68) by soft-agar colony formation assay. *P < 0.05; **P < 0.01; ***P < 0.001.

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