Fig. 5: The miR-200 family prevents cellular senescence by inhibiting multiple signaling pathways.
From: Complete loss of miR-200 family induces EMT associated cellular senescence in gastric cancer
A Selected secretory proteins identified from RNA-seq analysis were verified using ELISA by harvesting supernatants from 2-day cell culture. B The schematic representation for small molecule inhibitors (Inh) was used to investigate the pathways potentially mediating senescence in miR-200 family deficient cells. These pathway inhibitors including TGF-β (RepSox, 1 μM), TNF-α (TNF-α-IN-1, 1 μM), m-TOR (Rapamycin, 10 nM), Jak (CYT387, 1 μM), NF-κb (BAY11-7085, 1 μM) and were renewed in the media every 2 days. C SA-β-Gal staining for the miR-200 FKO clone A-31 treated with DMSO vehicle or a variety of drugs inhibiting different signaling pathways for 6 Days. Scale bar, 50 μm. D The percentage of cells staining positive for SA-β-Gal from C. E GDF-15. F CHI3L1 in culture media from A-31 cells following the treatment of specific inhibitors for 2 days was measured using ELISA. Data represent the mean ± SEM of triplicate independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001.
