Fig. 1: DAB2IP prevents premature mitotic exit and maintains MCC stability. | Oncogene

Fig. 1: DAB2IP prevents premature mitotic exit and maintains MCC stability.

From: Mitotic phosphorylation of tumor suppressor DAB2IP maintains spindle assembly checkpoint and chromosomal stability through activating PLK1-Mps1 signal pathway and stabilizing mitotic checkpoint complex

Fig. 1

DAB2IP-proficient and -deficient C4-2 (C4-2 D2 and C4-2 Neo cells) (A) and PC3 (PC3 siCtrl cells and siD2) (B) cells were synchronized in prometaphase using nocodazole (50 ng/ml) and then released into fresh media. Cells were collected at the indicated times after release. The expression of DAB2IP, Cyclin B1, Securin, and HSP70 were detected via immunoblotting. Cdc20 and APC/C complex were immunoprecipitated from nocodazole-blocked C4-2 D2 and Neo (C), and from DAB2IP-knockdown PC3 and its control (D) prometaphase cells lysates. The amounts of Mad2 and BubR1 binding with Cdc20 and Cdc27 were determined by immunoblotting.

Back to article page