Fig. 5: POU2F1 enhances the ALDOA promoter activity by directly binding to the ALDOA promoter. | Oncogene

Fig. 5: POU2F1 enhances the ALDOA promoter activity by directly binding to the ALDOA promoter.

From: The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity

Fig. 5

A Immunofluorescent analysis exhibited POU2F1 and ALDOA co-expression in SW620 and HCT116 cells (magnification x200, scale bars 50 μm). B Luciferase reporter assays indicated that POU2F1 enhanced the ALDOA promoter-controlled luciferase expression in the indicated cells. C Schematics of the POU2F1 putative binding sites in the 5′ upstream regions of the ALDOA promoter (−2000 bp ~ +50 bp). D Chromatin immunoprecipitation assay indicated that POU2F1 bound to the ALDOA promoter at sites 4, and 5. E Schematics of mutation strategies in the ALDOA promoter (−1000 bp ~ +50 bp). F Luciferase reporter assays exhibited that POU2F1 bound to the ALDOA promoter at site 5 to induce its expression. Data are representative images or expressed as the mean ± SD of each group of samples analyzed in triplicate from three separate experiments. **p < 0.01, ***p < 0.001.

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