Fig. 6: ALDOA is essential for the effect of POU2F1 on aerobic glycolysis and PPP in colon cancer cells. | Oncogene

Fig. 6: ALDOA is essential for the effect of POU2F1 on aerobic glycolysis and PPP in colon cancer cells.

From: The POU2F1-ALDOA axis promotes the proliferation and chemoresistance of colon cancer cells by enhancing glycolysis and the pentose phosphate pathway activity

Fig. 6

A Seahorse analysis of glycolysis in the indicated cells exhibited that POU2F1 enhanced glycolysis, dependent on up-regulating ALDOA expression. B–F The levels of glucose consumption and lactate production, G6PD activity, intracellular NADPH and the ratios of NADP+/NADPH in indicated cells. G Western blot analyses of the relative levels of glycolysis- and PPP-related protein expression in the indicated cells. H The levels of intracellular ROS in the indicated cells. I Western blot analyses of the relative levels of γ-H2AX and PCNA expression in the indicated cells. Data are representative images or expressed as the mean ± SD of each group of samples analyzed in triplicate from three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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