Fig. 2: ZNF154 functions as a tumor suppressor gene in ESCC and is downregulated by promoter hypermethylation. | Oncogene

Fig. 2: ZNF154 functions as a tumor suppressor gene in ESCC and is downregulated by promoter hypermethylation.

From: Targeted demethylation at ZNF154 promotor upregulates ZNF154 expression and inhibits the proliferation and migration of Esophageal Squamous Carcinoma cells

Fig. 2

A ZNF154 mRNA levels in the NATs (n = 9) and ESCC tissues (n = 5). B MR of ZNF154 promoter in ESCC cells treated with 5-Aza-dC (2 µM). C ZNF154 mRNA levels in ESCC cells treated with 5-Aza-dC. D ZNF154 mRNA and Myc-tag protein levels in Kyse30 and Kyse140 cells with or without ZNF154 overexpression. E Percentage of viable ESCC cells with or without ZNF154 overexpression. Data are expressed as absorbance. F Number of colonies formed by ESCC cells with or without ZNF154 overexpression. G Migration rates of ESCC cells with or without ZNF154 overexpression. Left panel: representative image of the Transwell assay; right panel: quantitative analysis. Experiments were repeated for two times with the same trend and the data presented are mean ± SD of one independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. Statistical analysis: Student’s t test (two sided). OE overexpression of ZNF154. NC and vectors are both negative controls.

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