Fig. 4: Targeted demethylation strategy in vivo. | Oncogene

Fig. 4: Targeted demethylation strategy in vivo.

From: Targeted demethylation at ZNF154 promotor upregulates ZNF154 expression and inhibits the proliferation and migration of Esophageal Squamous Carcinoma cells

Fig. 4

A Representative images of the tumors derived from Kyse-30 cells with targeted demethylation of ZNF154 promoter and ZNF154 overexpression. B, C Tumor growth curves of the indicated groups. D MR of the hypermethylated region at ZNF154 promoter following targeted demethylation. E, F ZNF154 mRNA levels in demethylation and overexpression groups. G The levels of Myc tag and GAPDH in the ZNF154 overexpression group. H, I Representative images of HE-stained lung metastatic nodules (magnification: left, 4×; right, 20×) and the number of metastatic nodules. Upper: Demethylation group (H); Lower: Overexpression group (I). Animal experiments were repeated for two times with the same trend and the data presented are mean ± SD of one independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. Statistical analysis: Student’s t test (two sided). Demethylation group: sgNC and sgs; Overexpression group: vector and ZNF154 OE. sg: sgRNA. sgs: sg1 + sg2 + sg3. sgNC sgRNA for negative control, OE overexpression of ZNF154.

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