Fig. 7: CST1 promotes distant metastasis in vivo. | Oncogene

Fig. 7: CST1 promotes distant metastasis in vivo.

From: CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1

Fig. 7

A Left: Images of peritoneal metastasis in nude BALB/c mice after injection of HGC-27-Vector/HGC-27-CST1/HGC-27-GPX4#sh cells into their abdominal cavity; images taken 60 days after injection. Right: statistical significance of the peritoneal nodules number and the ascites volume assessed by paired t-test, *p < 0.05, **p < 0.01. B Left: CST1-silenced MKN45 peritoneum xenograft tumors 30 days after injection. Right: statistical significance of the peritoneal nodules number and the ascites volume assessed by paired t-test, **p < 0.01, ****p < 0.0001. C Left: corresponding images of the lungs after injection of HGC-27-Vector/HGC-27-CST1/HGC-27-GPX4#sh cells by tail vein; images taken 3 months after injection. Right: statistical significance of the metastasis nodules number assessed by paired t-test, **p < 0.01. D Left: lung metastasis model of MKN45-shNC/MKN45-sh1-CST1/MKN45-sh2-CST1 8 weeks after tail vain injection. Right: statistical significance of the metastasis nodules number assessed by paired t-test, ***p < 0.001. E, F Left: HGC-27-Vector/HGC-27-CST1 and MKN45-shNC/MKN45-sh1-CST1/MKN45-sh2-CST1 cells were injected into tail vein; metastatic tumors in the livers were assessed 2 and 3 months after injection. Right: statistical significance of the metastasis nodules number assessed by paired t-test, **p < 0.01, ***p < 0.001. G Western blot analysis indicated metastasis tumors for GPX4 and CST1. H The content of MDA in the peritoneal metastatic tumor tissue of nude mice was detected, and the difference was statistically significant (***p < 0.001, ****p < 0.0001). HE-stained sections were magnified ×0.66 and ×5, respectively.

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