Fig. 2: An IRS1-PTEN axis is required for the proliferation of IGF1R-dependent GC cells. | Oncogene

Fig. 2: An IRS1-PTEN axis is required for the proliferation of IGF1R-dependent GC cells.

From: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

Fig. 2

A Western blot analysis of PTEN, p-Akt s473, t-Akt, p-IRS1 Y612, and t-IRS1 expression in BGC803 and MKN45 cells transfected with PTEN siRNAs. B Western blot analysis of IRS1, p-Akt t308, p-Akt s473, t-Akt, and PTEN expression in BGC803 and MKN45 cells infected with lentivirus expressing IRS1-specific shRNA or scramble shRNA. Cell proliferation ability detection of BGC803 and MKN45 cells after IRS1 knockdown by (C) CCK-8 assay, (D) plate clone formation assay, (E) soft agar clone formation assay, and (F) EdU assay. ***p < 0.001, **p < 0.01, *p < 0.05.

Back to article page