Fig. 4: Knockdown of IRS1 and NEDD4 does not affect the proliferation of IGF1R-independent GC cells. | Oncogene

Fig. 4: Knockdown of IRS1 and NEDD4 does not affect the proliferation of IGF1R-independent GC cells.

From: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

Fig. 4

A–C Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in AGS cells after silencing of IRS1 and NEDD4 and overexpressing NEDD4. Cell proliferation ability detection of AGS cells after IRS1 and NEDD4 knockdown and NEDD4 overexpression, respectively, by (D–F) CCK-8 assay, (G, H) plate clone formation assay, and (I–K) EdU assay. L Western blot analysis of p-Akt s473 and t-Akt in AGS cells after treatment of Heclin(10 μM) for 48 h. M Cell viability detection of AGS treated with Heclin (10 μM) by CCK-8 assay. ***p < 0.001, **p < 0.01, *p < 0.05, ns > 0.05.

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