Fig. 6: NEDD4 promotes proliferation of IGF1 signaling-dependent GC cells in a PTEN-dependent manner. | Oncogene

Fig. 6: NEDD4 promotes proliferation of IGF1 signaling-dependent GC cells in a PTEN-dependent manner.

From: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

Fig. 6

A, B Western blot analysis of NEDD4, PTEN, p-Akt s473, t-Akt, p-IRS Y612, and t-IRS1 protein level in BGC803 and MKN45 cells infected with lentivirus expressing scramble shRNA (control), shNEDD4-1 and shNEDD4-2 combined with PTEN siRNA. C, D CCK-8 assay and (E, F) EdU assay showing proliferation ability of the indicated cells. ***p < 0.001, **p < 0.01, *p < 0.05, ns p > 0.05.

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