Fig. 3: LZTFL1 suppresses cell proliferation in vitro and in vivo. | Oncogene

Fig. 3: LZTFL1 suppresses cell proliferation in vitro and in vivo.

From: LZTFL1 inhibits kidney tumor cell growth by destabilizing AKT through ZNRF1-mediated ubiquitin proteosome pathway

Fig. 3

a Western blots of the endogenous LZTFL1 in various ccRCC cell lines. GAPDH was used as loading control. b Western blots of LZTFL1 in ACHN and Caki1 cell lines transduced with lentiviruses expressing control vector (ACHN-NC, caki1-NC) or LZTFL1 (ACHN-LZTFL1, Caki1-LZTFL1), and in A498 cell line transduced with lentiviruses expressing control (A498-NC) or two different LZTFL1 shRNAs (A498-sh1 & A498-sh2). c Relative cell growth of ccRCC cell lines with control, LZTFL1-overexpressed or knocked down as indicated. Mean ± SD, ****, P < 0.0001, two-way ANOVA. d Colony forming ability of ccRCC cells with control, LZTFL1-overexpressed or knocked down as indicated. N = 3, mean ± SD, **, P < 0.01, ****, P < 0.0001, Student’s t test. e, f 5 × 106 cells with LZTFL1 overexpressed (e), knockdown (f), or corresponding control vectors were inoculated subcutaneously into the mice. Tumor volume was recorded weekly (left panel). Data are shown as mean ± SD, *, P < 0.05, ***, P < 0.001, two-way ANOVA. Tumor weight (middle panel) and tumor micrographs (right panel) at time of sacrifice were shown. mean ± SD, *, P < 0.05, **, P < 0.01, Student’s t test.

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