Fig. 4: EGR1 inhibits cell proliferation and causes cell cycle arrest by transcriptionally regulating p21cip1/waf1. | Oncogene

Fig. 4: EGR1 inhibits cell proliferation and causes cell cycle arrest by transcriptionally regulating p21cip1/waf1.

From: N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition

Fig. 4

A, B The mRNA and protein levels of EGR1 and p21cip1/waf1 upon EGR1 knockdown in MM cell lines RPMI-8226 and NCI-H929. C, D Cell proliferation was measured by EdU assay in RPMI-8226 and NCI-H929 cells transfected with siNC or siEGR1. (E, F) Cell cycle was detected by PI staining in RPMI-8226 and NCI-H929 cells transfected with siNC and siEGR1. G The EGR1-binding motif of p21 promoter (above) was predicted by JASPAR database. H, I ChIP assay was analyzed by RT-qPCR and agarose gel electrophoresis. J Luciferase reporter assays of wt- and mut-p21cip1/waf1 promoter sequences following EGR1 knockdown or YTHDF2 overexpression. Data are mean ± SD values. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. not significant.

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