Fig. 5: Silencing YTHDF2 suppresses cell proliferation via the EGR1/ p21cip1/waf1/CDK2-Cyclin E1 axis. | Oncogene

Fig. 5: Silencing YTHDF2 suppresses cell proliferation via the EGR1/ p21cip1/waf1/CDK2-Cyclin E1 axis.

From: N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition

Fig. 5

A, B The protein levels of the YTHDF2/EGR1/ p21cip1/waf1/CDK2-Cyclin E1 axis upon YTHDF2 knockdown (A) and EGR1 knockdown (B) in MM cell lines RPMI-8226 and NCI-H929. C, D Realtime-qPCR results of the EGR1 (C) and p21cip1/waf1 (D) mRNA expression upon silencing both YTHDF2 and EGR1 in RPMI-8226 and NCI-H929. E The protein levels of the YTHDF2/EGR1/ p21cip1/waf1/CDK2-Cyclin E1 axis upon silencing both YTHDF2 and EGR1 in RPMI-8226 and NCI-H929. F, G Cell cycle was detected by PI staining in RPMI-8226 and NCI-H929 cells simultaneously transfected with siYTHDF2 and siEGR1. H, I Cell proliferation was measured by EdU assay in RPMI-8226 and NCI-H929 cells simultaneously transfected with siYTHDF2 and siEGR1. Data are mean ± SD values. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. not significant.

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