Fig. 7: Overexpression of YTHDF2 enhances MM growth via the m6A-dependent degradation of EGR1 in vitro and in vivo. | Oncogene

Fig. 7: Overexpression of YTHDF2 enhances MM growth via the m6A-dependent degradation of EGR1 in vitro and in vivo.

From: N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition

Fig. 7

A, B The expression of the YTHDF2/EGR1/ p21cip1/waf1/CDK2-Cyclin E1 in RPMI-8226 and NCI-H929 transfected with LV-NC and LV-oeYTHDF2. C, D Cell proliferation was measured by EdU assay in RPMI-8226 and NCI-H929 transfected with LV-NC and LV-oeYTHDF2. E, F Cell cycle was detected by PI-staining in RPMI-8226 and NCI-H929 transfected with LV-NC and LV-oeYTHDF2. G RNA stability analysis of EGR1 after actinomycin D treatment upon YTHDF2 overexpression. H Methylated RNA immunoprecipitation of EGR1 transcripts in YTHDF2-upregulated MM cells. I RPMI-8226 cells transfected with LV-NC or LV-oeYTHDF2 were injected in the left or right flanks of BALB/c nude mice (n = 6). Mice were sacrificed when tumor diameter was more than 15 mm, xenograft tumors were completely dissected. J Tumor volume was recorded every 3 days and calculated by the formula: (width2 × length ×0.5). (K) Tumor weight was recorded after dissection. L Representative H&E staining in tumors from LV-NC (above) and LV-oeYTHDF2 (below) mice. M Representative IHC staining of YTHDF2, EGR1, p21cip1/waf1, CDK2, Cyclin E1 and Ki-67 in tumors from LV-NC and LV-oeYTHDF2 nude mice. Data are mean ± SD values. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. not significant.

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