Fig. 6: CircSPIRE1 expression was dependent on the QKI positive feedback loop. | Oncogene

Fig. 6: CircSPIRE1 expression was dependent on the QKI positive feedback loop.

From: Exosomal circSPIRE1 mediates glycosylation of E-cadherin to suppress metastasis of renal cell carcinoma

Fig. 6

A QKI was associated with the introns flanking circSPIRE1. RIP assays were conducted with a QKI antibody or control IgG, and primer sets covering the introns flanking circSPIRE1 (top). RNA enrichment indicates the levels of the indicated fragments in the anti-QKI precipitates relative to those in the IgG precipitates (bottom). Mean ± S.D. are provided. Dot plots reflect data points from three independent experiments. B Schematic structures showing RNA-binding sequence in the introns flanking the circSPIRE1 and a summary of intron truncations. C RNA pull-down assay performed to analyze the interaction between QKI and introns flanking circSPIRE1. D CircSPIRE1 expression upon QKI knockdown or overexpression. E Positive correlation between QKI expression and circSPIRE1 expression in 142 clinical samples of RCC. F Schematic diagram of mechanism of circSPIRE1 during RCC metastasis in RCC cells.

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