Fig. 4: Effects of BLACAT3 on BLCa angiogenesis and migration in vitro and in vivo.

HUVECs tube formation and transwell migration assay were performed to detect the effects of BLACAT3 knockdown on tumor angiogenesis and migration in T24 (A–C) and 5637 cells (D–F). Scale bars: 2 mm (black lines), 500 μm (orange lines). G, H In vivo imaging was performed on day 28 after tail vein injection of T24 cells with stable BLACAT3 knockdown into NSG mice, and fluorescent quantitative statistics were performed on representative images. I NSG mice were sacrificed on the 28th day after the lung metastasis model was constructed, and the gross lung samples were isolated, fixed, embedded and sliced, and then H&E staining was performed. Scale bars: 30 μm. J–L Anti-Ki67 and anti-CD31 immunofluorescent staining were performed to assess the T24 cell proliferation and angiogenesis in vivo. and fluorescent quantitative statistics were performed on representative pictures. Scale bars: 20 μm. Statistical significance was assessed using two-tailed Student’s t test between two groups, **P < 0.01, ***P < 0.001, ****P < 0.0001.