Fig. 3: CAFs reduce ER target gene expression in MCF7 cells via secreted factors. | Oncogene

Fig. 3: CAFs reduce ER target gene expression in MCF7 cells via secreted factors.

From: Cancer-associated fibroblasts rewire the estrogen receptor response in luminal breast cancer, enabling estrogen independence

Fig. 3

Induction of ER-α target genes PGR, CXCL12 and MYBL1 by estrogen (E2) in MCF7 cells in mono-culture or co-cultured with CAF2 for 48 h in transwells (TW) (A–C). Induction of the same genes of control (Ctrl) MCF7 cells or those that had previously been primed by CAF2 in transwells long-term (D–F). Transcription of ESR1 mRNA is reduced in long-term conditioned MCF7 cells (G). RNA levels are normalized to the untreated monocultures (Mo) or Ctrl MCF7 levels. E2 = estrogen. Unpaired ordinary one-way ANOVA, Fisher’s LSD multiple comparisons test: *P < 0.05, **P < 0.01, ***P < 0.001. Error bars: SEM.

Back to article page