Fig. 5: CAFs selectively modulate estrogen signaling. | Oncogene

Fig. 5: CAFs selectively modulate estrogen signaling.

From: Cancer-associated fibroblasts rewire the estrogen receptor response in luminal breast cancer, enabling estrogen independence

Fig. 5

Metascape analysis of pathway enrichment based on RNA-sequencing of MCF7 cells subjected to mono- or co-culturing with CAF2 cells in the presence or absence of E2 (A). The pathways listed are statistically significant and selected representative examples from the results of analysis after either 6 h or 24 h of E2 stimulation (for full list of enriched pathways, see STables 3 and 4). A 94-gene signature composed of the genes downregulated by CAF2 in MCF7 cells by > 1.5 fold change served as a predictive biomarker for poor response to endocrine treatment in patients with ER-α+ tumors (n = 2279) [67] (B). Similarly, the predictive capacity of the gene signature was validated in the TCGA dataset (C). Regulation of key individual genes using RNA-Seq-derived Transcripts Per Million (TPM) values in MCF7 cells derived from mono- or co-cultures with CAF2 cells (D, F), and with or without 24 h stimulation with E2 (E, G). Represented as relative levels of untreated mono-cultures and displaying the mean with standard deviation. E2= estrogen; Mo = mono-culture; Co = co-culture.

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