Fig. 5: USP36 promotes CRC proliferation and metastasis in vitro. | Oncogene

Fig. 5: USP36 promotes CRC proliferation and metastasis in vitro.

From: USP36 promotes colorectal cancer progression through inhibition of p53 signaling pathway via stabilizing RBM28

Fig. 5

A, B Transfection efficiency of USP36 in HCT 116 and LoVo cells, detected by qRT-PCR and WB. C, D CCK8 assays were applied to detect the viability of USP36 knockdown or overexpression cells. E, F Colony formation assays were used to evaluate the proliferation of CRC cells. G, H Flow cytometry was used to analyze the apoptosis rates (LR + UR) of cells. LR, early apoptotic cells; UR, terminal apoptotic cells. I, J Wound healing assays were used to assess cell migration ability. K, L Transwell assays were performed to evaluate the migration and invasion abilities of CRC cells. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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