Fig. 7: USP36 positively correlates with RBM28 in CRC samples.

A Western blot analysis of cell lysates from 24 paired CRC samples using USP36 and RBM28 antibodies. B Correlation analysis of USP36 and RBM28 in CRC samples (n = 24). The chi-square test was applied to statistical analysis. Pearson R indicates the correlation coefficient. C, E The protein level of USP36 and RBM28 was detected by IHC in the TMA of 80 paired CRC patients’ samples (left panel). Representative images of high USP36 and RBM28 expression in CRC tissues (right panel). D, F The IHC score of USP36 and RBM28 in the TMA of 80 paired CRC patients’ samples. The comparison between cancer tissues and matched adjacent tissues was conducted using either the Wilcoxon unpaired test (left panel) or paired t-test (right panel). G Representative images of IHC staining of simultaneous high or low expression of both USP36 and RBM28 on the tissue of CRC patients. H USP36 IHC-scores (X-axis) positively correlate with RBM28 IHC-scores (Y-axis) in CRC samples (n = 80). The p value was calculated from a linear regression analysis. R is the correlation coefficient. I, J Kaplan–Meier plots of OS in the TMA of 80 paired CRC patients, divided into “high” and “low” expression groups based on the median IHC score of USP36 and RBM28. The p value was calculated from a log-rank test. HR hazard ratio. All data are presented as the means ± SD of three independent experiments. ***p < 0.001.