Fig. 5: FBL facilitates short-patched base excision repair pathway. | Oncogene

Fig. 5: FBL facilitates short-patched base excision repair pathway.

From: Enhancing low-dose radiotherapy efficacy with PARP inhibitors via FBL-mediated oxidative stress response in colorectal cancer

Fig. 5

A The schematic illustration of BER efficiency assay (left). The BER efficiency assay (the ratio of GFP+ cells to DsRed+ cells analysed by FACS after 48 h transfection) in shctrl or shFBL (#1, #2) RKO cell lines (right) (**p < 0.01, error bar = ±SEM, n = 3). B The BER assay in shctrl, shFBL RKO cells stably expressing Flag empty vectors or shFBL RKO cells rescued by shFBL resistant Flag-FBLR. (*p < 0.05, **p < 0.01, error bar = ±SEM, n = 3). The recruitments (left) and intensity analyses (right) of EGFP-Lig III C EGFP-FEN1 D and EGFP-PCNA E recruited to the DNA damage sites induced by 405 nm microirradiation in shFBL#2 RKO cells stably expressing Flag-empty, Flag-FBLR or Flag-FBLΔGAR R, respectively. (*p < 0.05, **p < 0.01, error bar = ±SEM, n = 10–15 cells, scale bar = 2.5 μm).

Back to article page