Fig. 2: IPF fibroblasts significantly enhanced the aggressiveness of NSCLC cells in vitro. | Oncogene

Fig. 2: IPF fibroblasts significantly enhanced the aggressiveness of NSCLC cells in vitro.

From: Senescent lung fibroblasts in idiopathic pulmonary fibrosis facilitate non-small cell lung cancer progression by secreting exosomal MMP1

Fig. 2

A A schematic diagram illustrating the co-culture model. (Created with Figdraw). B Morphological changes of A549 and SK-MES-1 cells following co-cultivation with normal human lung fibroblasts (NHLFs) and diseased human lung fibroblasts (DHLFs). Scale bar, 100 µm. C, D Cellular proliferation of A549 (C) and SK-MES-1 (D) after co-culture with NHLFs and DHLFs measured by CCK8 assay. E, F Colony formation assays assessing clonogenicity of A549 and SK-MES-1 cells after co-culture with NHLFs or DHLFs. G–J Transwell assays evaluating migration and invasion abilities of A549 (G, H) and SK-MES-1 (I, J) cells after co-culture. Scale bar, 100 µm. K–M Immunoblotting analysis of EMT markers (E-cadherin, N-cadherin, Vimentin, and Snail). Representative blots shown in (K), with statistical analysis plots of protein levels in (L) and (M). (Results are presented as means ± SD, n = 5, *p < 0.05; **p < 0.01; *** p < 0.001).

Back to article page