Fig. 3: Exosomes from IPF fibroblasts promote NSCLC cell proliferation. | Oncogene

Fig. 3: Exosomes from IPF fibroblasts promote NSCLC cell proliferation.

From: Senescent lung fibroblasts in idiopathic pulmonary fibrosis facilitate non-small cell lung cancer progression by secreting exosomal MMP1

Fig. 3

A A schematic diagram illustrating the differential ultracentrifugation process for isolating supernatant components. (Created with Figdraw). B Western blot analysis detecting Annexin V (microvesicle marker) and CD9, CD63 (exosome markers), confirming successful extraction. C, D The CCK8 assay assessing proliferation of A549 (C) and SK-MES-1 (D) after incubation with various supernatant components, including exosomes, microvesicles, and EVs-free supernatant. E, F Colony formation assays evaluating the colony-forming ability of lung cancer cells incubated with exosomes, microvesicles, or EVs-free supernatant. G TEM images of exosomes secreted by normal human lung fibroblasts (NHLF) and diseased human lung fibroblasts (DHLF). Scale bar, 100 nm. H Size distribution of exosomes measured via nanoparticle tracking analysis. I Fluorescent DiO-labeled exosomes (green) confirming internalization by recipient cells. Scale bar, 50 µm. J, K CCK8 assays evaluating A549 cells (J) and SK-MES-1 cells (K) proliferation following incubation with NHLF- and DHLF-derived exosomes. L, M Representative images (L) and histogram analysis (M) of colony formation after exosome incubation. (Results are presented as means ± SD, n = 3, *p < 0.05; **p < 0.01; *** p < 0.001).

Back to article page