Fig. 4: Mass spectrometry analysis reveals upregulated senescence-associated secretory phenotype (SASP) factors in DHLF-exosomes. | Oncogene

Fig. 4: Mass spectrometry analysis reveals upregulated senescence-associated secretory phenotype (SASP) factors in DHLF-exosomes.

From: Senescent lung fibroblasts in idiopathic pulmonary fibrosis facilitate non-small cell lung cancer progression by secreting exosomal MMP1

Fig. 4

A Volcano plots illustrating the differential expression of proteins identified by mass spectrometry between exosomes derived from normal human lung fibroblasts (NHLF) and diseased human lung fibroblasts (DHLF). B Gene Ontology enrichment analysis of the upregulated proteins, visualized with bar plots. (Top 5 per classification). C KEGG pathway enrichment analysis of the top 30 upregulated proteins visualized with a bubble diagram. D A heat map showing relative expression levels of SASP factors in NHLF-exosomes and DHLF-exosomes. E RT-qPCR analysis of mRNAs encoding SASP factors including IL-6, MMP1, MMP3, FBLN2, IGFBP3, IGFBP4. F Kaplan–Meier survival analysis curve of low MMP1 and high MMP1 patients derived from 482 patients with NSCLC in TCGA (https://tcga-data.nci.nih.gov/tcga/). TCGA, the cancer genome atlas (Results are presented as means ± SD, n = 3, *p < 0.05; **p < 0.01; *** p < 0.001).

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