Fig. 8: YBX1 promoted osteosarcoma progression in vitro and in vivo through DDX3X. | Oncogene

Fig. 8: YBX1 promoted osteosarcoma progression in vitro and in vivo through DDX3X.

From: KPNA2 promotes osteosarcoma progression by regulating the alternative splicing of DDX3X mediated by YBX1

Fig. 8

A–C Colony formation assays detected cell proliferation of U2OS and MNNG/HOS cells (n = 4). D, E Transwell assays demonstrated the migration and invasion capabilities of U2OS and MNNG/HOS cells. The scale bar was 100 µm. F The tumor growth curve (n = 5). G MNNG/HOS cells were subcutaneously into the nude mice for xenografts assay (n = 5). H Ki-67 staining of the tumor. The scale bar was 100 µm. I Excised tumor mass as indicated (n = 5). J The bar chart showed the quantitative results of Ki-67. K Flow cytometry results that shDDX3X reversed the effect of shYBX1 on G0/G1 cell cycle in osteosarcoma cells (n = 3). L Western blotting analysis of osteosarcoma tumor cells (U2OS and MNNG/HOS) after transfected with indicated lentiviral particles. Data were shown as the mean ± standard deviation of at least three independent experiments. ns no statistical significance; *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. One-way analysis of variance (ANOVA) was used to compare the difference in (B, C, E, F, I, J). Data were presented as mean ± SD (error bars) and representative of at least three independent experiments in (A–L).

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