Fig. 6: OE19-PS and OE19-R cells display increased levels of reduced glutathione.
From: NRF2-mediated persistent adaptation of oesophageal adenocarcinoma cells to HER2 inhibition
A Schematic representation of critical enzymes involved in glutathione metabolism. Red arrows indicate the genes encoding the enzymes upregulated in OE19-PS (treated with lapatinib for 35 days). B Detection of GCLM transcript levels in OE19-PT, OE19-PS and OE19-RT1 cells by qPCR (n = 3). C ChIP-qPCR shows endogenous binding of NRF2 to the GCLM promoter region (−43 to −203) in OE19-PS cells. Binding following immunoprecipitation with immunoglobulin (Ig) G control is also shown. (n = 2). D, E The amount of reduced glutathione (GSH) was measured by flow cytometry (n = 4). F Kaplan–Meier plot survival curves comparing subjects in the TCGA OAC cohort based on risk scores established from three NRF2 core target genes. Samples were divided into two groups, high (red) and low (blue), based on the median value of risk scores. Cox regression was used to calculate the hazard ratio (HR) and the p-value. Statistical analyses were performed by one-way ANOVA (B) or unpaired t-test (D, E).
