Fig. 3: SUMOylation does not affect stability of α-catenin and cellular localization.
From: α-catenin SUMOylation increases IκBα stability and inhibits breast cancer progression
a MDA-MB-231 cells transfected with indicated plasmids were stained with Flag antibody as previously described52. b MDA-MB-231 cells infected with indicated virus. Nuclear and cytoplasmic extraction were detected using western blot. Fibrillarin and GAPDH were measured to normalize protein abundance of α-catenin, respectively. c HEK293T cells were transfected with indicated plasmids. Cytosolic and nuclear fractions were inmmunoprecitated using Flag gel. Purified cell extracts were tested by western blot. d HEK293T cells transfected with WT Flag-α-catenin or K870R were treated with 50 mg ml−1 CHX, and cell lysates were detected using western blot
