Fig. 4: α-catenin SUMOylation inhibits NF-κB pathway.
From: α-catenin SUMOylation increases IκBα stability and inhibits breast cancer progression
a HEK293T cells were transfected with indicated plasmids. Luciferase activity was measured with or without pre-treating of the cells with 10 ng ml−1 TNF-α 4 h (for pNFκB luciferase). b MDA-MB-231 and MDA-MB-157 cells transfected with wild-type α-catenin or its mutant K870R and then subjected to Western blot by anti-α-catenin or anti-IκBα antibody, respectively. c Real-time PCR was used to measure the mRNA levels of NFκB target genes TNF-α, IL-8, VEGF and uPA in MDA-MB-157 cells stable transfected with wild-type α-catenin or its mutant K870R. n = 3 wells per group
