Fig. 5: SUMOylation enhances α-catenin interacting with IκBα.

a MCF-7, T47D, and MDA-MB-231 cell lysates were immunoprecipitated with anti-α-catenin antibody or control IgG followed by Western blot with anti-SUMO1 antibody. b MDA-MB-157 cells transfected with wild-type α-catenin or its mutant K870R and then subjected to inmmunoprecipitation with anti-α-catenin antibody followed by Western blot with anti SUMO1 antibody. c MCF-7 and MDA-MB-231 cells were transfected with α-catenin-shRNA or its control vector followed by Western blot with anti-α-catenin or anti-IκBα antibody, respectively. d MDA-MB-231 cells treated with or without 10 ng ml⁻¹ TNF-α 15 min before lysis, and then lysates were immunoprecipitated with anti-α-catenin antibody or control IgG followed by Western blot with anti-SUMO1 antibody. e MDA-MB-231 cells transfected with wild-type α-catenin or its mutant K870R and then subjected to inmmunoprecipitation with anti-α-catenin antibody followed by Western blot with anti-IκB-α antibody. f In the same condition as e. g HEK293T cells were transfected with indicated plasmids. Cells were treated with 10 μM MG132 and 20 ng ml⁻¹ of TNF-α for 30 min, and IκB-α was purified with anti-Flag gel followed by Western blot with HA or anti-Flag antibody. “*” non-specific bands