Fig. 2: Generation and characterization of conditional knock-in transgenic mouse models of skin expressing MYCWT or MYCT58A. | Oncogenesis

Fig. 2: Generation and characterization of conditional knock-in transgenic mouse models of skin expressing MYCWT or MYCT58A.

From: Altering MYC phosphorylation in the epidermis increases the stem cell population and contributes to the development, progression, and metastasis of squamous cell carcinoma

Fig. 2

a Knock-in strategy for conditional expression of MYCWT-HA and MYCT58A-HA. Rosa-Floxed-Stop (RFS)-Myc constructs are shown. Arrowheads indicate loxP sites. bpA is a transcription stop sequence. Inserted cMyc cDNAs have a C-terminal HA tag. The K5-CrePR transgene encodes a fusion protein consisting of Cre recombinase and a truncated progesterone receptor driven by the Keratin 5 (K5) promoter. Cre recombination allows ectopic MycWT or T58A expression driven from the ROSA promoter. b Recombined allele was detected in skin from MYCWT and MYCT58A mice after RU486 treatment. The control is RFS-MycWT without K5-CrePR after RU486 treatment. c PCR showing ectopic and total Myc mRNA expression in MYCWT or MYCT58A mice compared to the control (no Cre) mice 8 weeks after RU486 induction. d Representative images showing expression of ectopic MYC by HA staining in normal skin tissues of Ctrl, MYCWT, MYCT58A mice 8 weeks after RU486 treatment.

Back to article page