Fig. 4: MYCT58A expression results in increased proliferation and reduced apoptosis after DMBA/TPA chemical carcinogenesis treatment. | Oncogenesis

Fig. 4: MYCT58A expression results in increased proliferation and reduced apoptosis after DMBA/TPA chemical carcinogenesis treatment.

From: Altering MYC phosphorylation in the epidermis increases the stem cell population and contributes to the development, progression, and metastasis of squamous cell carcinoma

Fig. 4

a, b TUNEL staining (a) and quantitation (b) to assess apoptosis in the epidermis and hair follicle of mice 72 h post DMBA/TPA one-time treatment. Scale bar = 50 µm. Shown is mean and SD of 5 mice per genotype. p-value is from a one-way ANOVA, followed by Tukey’s multiple comparison test, ***p < 0.001. c, d BrdU staining (c) and quantitation (d) to assess proliferation in the epidermis and hair follicle of mice 48 h post DMBA/TPA one-time treatment. Scale bar = 100 µm. Shown is mean and SD of 3 mice per genotype. p-value is from a one-way ANOVA, followed by Tukey’s multiple comparison test, ns = not significant. e, f BrdU staining (e) and quantitation (f) in the epidermis adjacent to the skin lesions after 8 weeks of DMBA/TPA treatment of mice from each genotype. Scale bar = 50 µm. Shown is mean and SD of 5 mice per genotype. p-value is from a one-way ANOVA, followed by Tukey’s multiple comparison test, **p < 0.01, ***p < 0.001. g, h TUNEL staining (g) and quantitation (h) to assess apoptosis in the epidermis and hair follicle of mice 8 weeks post DMBA/TPA treatment. Scale bar = 100 µm. Shown is mean and SD of 3 mice per genotype. p-value is from a one-way ANOVA, followed by Tukey’s multiple comparison test, ns = not significant.

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